Xenbase Image ID: 128563

Figure 5. The ability to down-regulate zic3 and irx1 is lost in the GARP mutant.(A) The FoxD4L1 C-terminal mutant expressing clones, marked by nuclear β-Gal (pink dots), are located in the neural ectoderm. For L>A, Q>R and GARG mutants, the βGal labeled cells are less intensely stained (blue) than their neighboring cells (e) expressing endogenous levels of zic3 or irx1. For GARP, the βGal labeled cells are stained at the same intensity as the neighboring cells (e). Insets are higher magnifications of the clone, the position of which is indicated on the whole embryo by a bracket. For zic3, images are dorsal views with vegetal pole towards the bottom; for irx1, images are frontal views with dorsal towards the top. (B) The percentage of embryos in which the FoxD4L1 C-terminal mutants caused down-regulation of zic3 or irx1 in the dorsal neural ectoderm. Numbers on each bar indicates sample size; * indicates significant difference from wild type (WT) at the p<0.001 level. Data for WT, ΔRII-Cterm and A6 are from [39]. Image published in: Klein SL et al. (2013) Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder. Larger Image Printer Friendly View

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