Xenbase Image ID: 128564

Figure 6. FoxD4L1 mutant proteins have access to the nucleus in a pattern similar to wild-type FoxD4L1.(A) Left panel: epifluorescence image of wild-type, myc-tagged FoxD4L1 protein in neural ectoderm of stage 13 embryo. Tagged protein is in the cytoplasm and in the nucleus (arrows). Middle panel: confocal image of a similar sample shows that the protein (green) is localized in the periphery of the nucleus (blue) where chromatin is concentrated in non-mitotic cells. Right panel: example from a similar sample in which a 32-channel signature spectral curve analysis was performed. Red pixels around the periphery of the nucleus represent sites of DNA (blue) and protein (green) colocalization. (B) Left panel: DAPI nuclear staining of cells in the superficial neural ectoderm of stage 12 embryo. Middle panel: Myc-tagged GARP protein (green), like wild-type protein, is found in the cytoplasm and in the periphery of the nucleus. Right panel: a 32-channel signature spectral curve analysis was performed to demonstrate with confidence nuclear localization of the tagged protein. Magenta pixels represent sites of DNA (blue) and protein colocalization. (C) Left panel: DAPI nuclear staining of cells in the deep layer of the stage 14 neural plate. Middle panel: Myc-tagged AB4 protein (green) also is found in the cytoplasm and in the periphery of the nucleus. Right panel: a signature spectral curve analysis was performed: magenta pixels represent sites of DNA (blue) and protein colocalization. White bars indicate 7 µm. Image published in: Klein SL et al. (2013) Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder. Larger Image Printer Friendly View

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