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FIGURE 3. (A) Comparison of the effects of applying 100 μL frog Ringer’s solution containing 3. 3% DMSO vs. applying the same solution plus 10 mM 2-APB to uninjected oocytes (gray bars) or oocytes injected with cRNA coding for hKCNK2 (light green bars) and hKCNK10 (dark green bars) (n = 3–5 oocytes/group). The time of exposure was approximately 10 s. (B) Top: Representative voltage clamp recording from an oocyte expressing hKCNK2 exposed to vehicle solution (green rectangles) or the same solution plus 10 mM DMSO (blue rectangles). Bottom: Representative voltage clamp recording from an inside-out patch-clamp of a HEK293 cell expressing hKCNK10, before and after exposure to 1% DMSO (light green rectangle). (C) hKCNK4 currents recorded from a transfected HEK293 cell before (right) and after (left) the application of 300 μM 2-APB. Currents were elicited by voltage pulses from -110 to +50 mV in 20 mV steps, 400 ms in duration, from a holding potential of -60 mV. Image published in: Beltrán L et al. (2013) Copyright © Beltrán, Beltrán, Aguado, Gisselmann and Hatt. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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