Xenbase Image ID: 128743

Figure 3. Twist and Cxcr4, but not Snail1 or Snail2, are Hif-1α targets in the neural crest. (A–L) Xenopus embryos. In situ hybridization for Snail1, Snail2, Twist, and Cxcr4 after ATGMOhif-1α injection. Asterisks show the injected side. (A–F) Snail1 and Snail2 expression. Note that neither Snail1 nor Snail2 are affected by inhibition of Hif-1α, but a clear inhibition of neural crest migration is observed. (G–I) Twist expression is lost after ATGMOhif-1α injection (arrows). (J–L) Cxcr4 expression. (J) Note that strong expression in the neural plate is not affected, whereas the neural crest expression is visible only in the right (noninjected) side as three masses next to the neural plate. Arrows indicate absence of gene expression in the neural crest at the injected side. (M and N) Quantification of results shown in A–L. Bars represent the standard deviation from three independent experiments, a minimal of 40 embryos was analyzed for each case. (O and P) Quantitative PCR (O) and RT-PCR (P) of neural crest dissected from embryos injected with a control MO or with ATGMOhif-1α genes analyzed as indicated. EF-1α was used as a loading control. Bars represent the standard deviation from three independent experiments. A minimal of 30 embryos was analyzed for each case. ***, P < 0.005. Image published in: Barriga EH et al. (2013) © 2013 Barriga et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Gene Synonyms Species Stage(s) Tissue snai1.S LOC108702592, sna, snail, snail1, xSna, Xsnail X. laevis Throughout NF stage 17 neural crest cranial neural crest premigratory neural crest cell snai1.S LOC108702592, sna, snail, snail1, xSna, Xsnail X. laevis Throughout NF stage 25 neural crest cranial neural crest postmigratory neural crest cell mandibular crest snai2.L slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. laevis Throughout NF stage 17 neural crest cranial neural crest premigratory neural crest cell snai2.L slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. laevis Throughout NF stage 25 neural crest cranial neural crest postmigratory neural crest cell twist1.S twist, twist1-a, twist1-b, X-twi, Xtwi, Xtwist X. laevis Throughout NF stage 17 neural crest cranial neural crest twist1.S twist, twist1-a, twist1-b, X-twi, Xtwi, Xtwist X. laevis Throughout NF stage 25 neural crest cranial neural crest postmigratory neural crest cell mandibular crest cxcr4.L cd184, cxcr4-a, cxcr4-b, fb22, hm89, hsy3rr, lap3, lcr1, lestr, npy3r, npyr, npyrl, npyy3r, xcxcr4 X. laevis Throughout NF stage 17 neural crest cranial neural crest pre-chordal neural plate chordal neural plate cxcr4.L cd184, cxcr4-a, cxcr4-b, fb22, hm89, hsy3rr, lap3, lcr1, lestr, npy3r, npyr, npyrl, npyy3r, xcxcr4 X. laevis Throughout NF stage 25 neural crest eye neural tube postmigratory neural crest cell Image source: Published Larger Image Printer Friendly View

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