Xenbase Image ID: 128747

Figure 7. Hif-1α controls cell dispersion in a Twist-dependent manner. (A–D) In situ hybridization against the neural crest marker Snail2. (A) Injection of ATGMOTwist into the prospective neural crest at the 16- and 32-cell stage embryo does not affect neural crest induction. Asterisk shows the injected side. (B–D) Injection of ATGMOTwist blocks neural crest migration. (E–S) Analysis of dispersion of cultured neural crest cells as described in Fig 6. (E–J) Nuclear labeling is shown at time 0 and after 3 h. (K–M) Delaunay triangulation shown for 3 h time to analyze the distance between neighbor cells. Color coded according to the area of the triangles after the indicate treatments. (N–P) Bright-field images after the indicated treatments. (Q–S) Membrane-GFP merged with nuclear-RFP to observe cell morphology. (T) Quantification of Delaunay triangulation for all the conditions analyzed. ***, P < 0.005. Errors bars represent standard deviation of three independent experiments, with a minimal of 60 cells analyzed for each condition. (U) Percentage of explants exhibiting cell dispersion. Image published in: Barriga EH et al. (2013) © 2013 Barriga et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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