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Figure 7. Loss of Sox4/11 function leads to cell apoptosis in the developing eye.A: TUNEL staining of Sox4 or Sox11 depleted embryos at stage 32. The areas where apoptotic cells have been counted are highlighted by a dotted circles and increased cell apoptosis was labeled by arrows. B: Quantitative representation of the TUNEL staining shown in A. n = number of independent experiments. **** P≤0.0001. C: Increased caspase 3/7 activity after loss of Sox4 or Sox11 at stage 32. Values represent relative light units (RLU) normalized to Control MO injected embryos. n = number of independent experiments. Error bars indicate standard error of the means (s.e.m.). D: Unilateral injection of 40 ng Sox4 or Sox11 MO led to increased caspases 3/7 activity while uninjected sides or Control MO injected embryos were not affected. Values represent relative light units (RLU) normalized to the uninjected side. n = number of independent experiments. Error bars indicate standard error of the means (s.e.m.). E: Increased cell apoptosis upon loss of Sox4 and Sox11 was rescued by tp53 inhibition. F: A quantitative representation of the results in E is given. n = number of independent experiments, N = number of injected embryos analyzed. G: Crosssections of Sox4 or Sox11-depleted embryos demonstrating mitotic cells (blue, pH3 staining). Black arrowheads point to the MO injected side. H: A quantitative representation of the data in F is given. Sox4 or Sox11 depletion had not significant (n.s.) effect on cell proliferation in the eye at stage 32. n = number of independent experiments. Error bars indicate standard error of the means (s.e.m.), n.s. not significant, * P≤0.05, **** P≤0.0001. Image published in: Cizelsky W et al. (2013) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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