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Figure 5. XLHMGA2βa, but not XHMG-AT-hook1, interacts with the same molecular partners of mammalian HMGA.(A) Blue coomassie stained analysis of different HMGA molecular partners produced as GST-fused protein and of GST alone. PR: pocket region; CT: C-terminal region; ZnF: Zinc finger region. (B) GST-pull down assays performed with the GST-fused HMGA molecular partners shown in panel A and IVT and [35S]-methionine radiolabeled XHMG-AT-hook1 (HA–XATH1), hHMGA2 (HA–hA2), and XLHMGA2βa (HA–XLA2βa). For each IVT protein used input is shown in lanes 1, 6, and 10 (10% of the amount used in GST-pull down experiments). GST alone is used as a negative control.

Image published in: Macrì S et al. (2013)

Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license

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