Xenbase Image ID: 128975

Figure 3. Pharmacologically lowering ROS impairs tail regeneration. (a) Panels show representative HyPerYFP imaging and quantification of tadpole tails treated with DMSO, 2μM DPI, 200μM apocynin (APO), or 200μM MCI-186 (MCI) treatments at 12 hours after amputation. (b) Representative tails and quantification of regenerated tail length at 72 hours after amputation following the indicated inhibitor treatments. (c) Images and quantification of tadpoles that were exposed to DMSO, DPI, APO, or MCI (same doses as above) from 0-72hpa, and then cultured in normal media until 7dpa. Error bars indicate standard deviation of the mean (s.d.) of (n) specimens. Red lines indicate initial point of amputation. Significance was determined using one-way ANOVA versus DMSO control. **, P < 0.01; ***, P < 0.001; no significance (n.s.), P > 0.05. Image published in: Love NR et al. (2013) Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder. Larger Image Printer Friendly View

Return to previous page