Xenbase Image ID: 128976

Figure 4. Morpholino mediated knockdown of cyba results in lowered amputation-induced ROS production and decreased regenerative tissue formation. (a) Western-blot against the FLAG epitope in cyba-flag mRNA injected cyba morphant or control embryos. (b) Western-blot against the myc epitope in myc-cyba mRNA injected cyba morphant or control embryos. (c) Representative transillumination and HyPerYFP imaging at amputation (T0) and 24 hpa in control, cyba morphants, and myc-cyba rescue cyba morphants, showing inhibition of ROS production and regenerative tissue formation by the cyba MO and rescue by co-injection of the MO-resistant myc-cyba mRNA. Black closed arrow shows regenerative bud. The quantification of HyPerYFP ratio increases following amputation in control, cyba morphants, and myc-cyba rescue construct injected cyba morphants is shown to the right of the panels. (d) Quantification of regenerative bud formation in control, cyba morphants, and myc-cyba rescue construct injected cyba morphants. Error bars indicate standard deviation (s.d.) of (n) specimens. Significance was determined using one-way ANOVA versus control. **, P < 0.01; ***, P < 0.001. Image published in: Love NR et al. (2013) Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder. Larger Image Printer Friendly View

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