XB-IMG-128977
Xenbase Image ID: 128977
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Figure 5. Amputation induced ROS are important for proper growth factor signaling during tail regeneration. (a) Representative images and quantification of X. laevis tadpole tail mitotic cells at 36hpa when cultured in control (DMSO) or ROS inhibitors DPI (2μM), APO (200μM), and MCI (200μM). (b) Representative images and relative Wnt/β-catenin signaling reporter dsGFP fluorescence at 36hpa in control (DMSO) or ROS inhibitors DPI (2μM), APO (50μM), and MCI (200μM) treated X. tropicalis tadpoles. (c) RT-PCR reactions amplifying fgf20 or reference gene rpl8 in control (DMSO) or ROS inhibitors DPI (2μM), APO (50μM), and MCI (200μM) at 36hpa in X. tropicalis tadpoles. (d)
In situ hybridization of fgf20 in DMSO controls versus DPI treated X. tropicalis tadpole tails during the tail regrowth phase of tail regeneration. (e) RT-PCR reactions detecting fgf20 in X. tropicalis control or fgf20 morphants. (f) Representative regenerated control or fgf20 morphant tadpole tails at 48hpa. (g) Quantification of regenerated tail length in control or fgf20 morphants. Error bars indicate standard error of the mean (s.e.m.) of (n) specimens. Significance was determined using one-way ANOVA or unpaired t-tests. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Image published in: Love NR et al. (2013) Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder. Larger Image Printer Friendly View |