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Fig. 3. Effect of Myb deletion on initiation of FOXJ1 expression in the lung. (A-F) Mice carrying a conditional allele of Myb (Mybfl) and a Cre recombination reporter (mTmG) were crossed to mice carrying Shh-Cre or Nkx2.1-Cre transgenes to selectively delete Myb from the developing airway epithelium (Myb CKO), and lungs from E15.5 control (Myb+/fl) and Myb CKO mice were immunostained for the proteins indicated. (A,B) MYB immunostaining confirms loss of MYB expression in Myb CKO tissue. (C,D) FOXJ1 expression is not detected in Myb CKO tissue. (E,F) Large concentrations of PCNT, indicative of ciliating cells with amplified centrioles (inset in E), are not observed in Myb CKO tissue (inset in F). PCNT staining of centrosomes (arrowheads) is seen in both control and Myb CKO non-MCCs. (G,H) Lungs from E15.5 Foxj1 control heterozygous and homozygous knockout (Foxj1-/-) mice immunostained for MYB. MYB is expressed normally in the absence of Foxj1. Scale bars: 10 μm. Image published in: Tan FE et al. (2013) © 2013. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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