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Fig. 4. Effect of MYB on FOXJ1 expression in MTECs and a HEK293T cell cotransfection assay. (A,B) MTECs infected with a lentivirus expressing either a tdTomato-MYB fusion protein (tdT-MYB) or RFP alone were harvested at ALI +4 days and stained for RFP (A) or tdTomato (B) and FOXJ1. Scale bar: 20 μm. (C) Quantification of the percentage of infected cells that express FOXJ1. *P<0.0001 by chi-square test. (D) Luciferase activity driven by the Foxj1 reporter illustrated (nucleotides -876 to +162 with respect to the Foxj1 transcription initiation site) cotransfected into human HEK293T cells with pCDH empty vector (-) or pCDH constructs expressing the indicated proteins. Reporter activity is normalized to the vector-only (pCDH) control. *P<0.05, **P<0.003; n.s., not significant (Student’s t-test). Error bars indicate s.e.m. Image published in: Tan FE et al. (2013) © 2013. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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