Xenbase Image ID: 129223

Figure 3. Mechanics, anchoring and structural regulation of nuclear actina, Compressive forces were applied to the GV using a microneedle, demonstrating a coupled elastic response of the actin meshwork (Lifeact::GFP, green) and lamin cortex (RFP::Lamin B3, red). Arrowhead shows increased intensity, suggesting actin polymerization occurs in response to force. b, Tensile forces were applied to the GV using a microneedle, showing a similar coupled elastic response. c, Two nuclear bodies trapped in a dense native actin meshwork. d, Tropomyosin injection leads to a compacted actin meshwork, and the nuclear bodies are often deformed. e, Fascin injection leads to bundling of the actin meshwork. For c–e, nucleoli are labeled with NPM1::RFP (red), and actin is labeled with Lifeact::GFP (green). d, Bar graph showing the MSD of large (R=1 μm) beads at a lag time of 5 sec, under various conditions: untreated (n=35 z-positions from 14 GVs, 3,011 particles identified), apyrase (n=13 z-positions from 4 GVs, 617 particles identified, p-value = 0.22), tropomyosin (n=24 z-positions from 12 GVs, 1,278 particles identified, p-value = 0.16), alpha-actinin (n=18 z-positions from 5 GVs, 1,254 particles identified, p-value = 0.05), and fascin (n=39 z-positions from 18 GVs, 2,123 particles identified, p-value = 0.34). Error bars = s.e.m. Scale bar is 10 μm in all images. Image published in: Feric M and Brangwynne CP (2013) Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder. Larger Image Printer Friendly View

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