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Figure 3. Comparison of de novo transcriptome assemblies.A. Transcripts assembled by the Abyss assembler from oligo-dT and Cap-captured mRNA libraries were aligned to the X. tropicalis ENSEMBL annotated transcripts using BlastX. The number of ENSEMBL transcripts that were matched by each assembler:library pair are represented as a Venn Diagram. The number of transcripts present in each assembler:library pair are listed in Table 2. Because multiple sequences from each de novo assembly align to X. tropicalis genes these numbers are omitted from the figure for the sake of simplicity. B. Same comparison as in A, except that Velvet was used as the assembler instead of Abyss. C. Transcripts assembled by Abyss or Velvet from Cap-capture mRNA libraries were aligned to the X. tropicalis ENSEMBL transcripts using BLAT. Unique and common ENSEMBL transcripts are represented by a Venn Diagram D. Same comparison as in C, except that dT libraries are compared instead of Cap-captured libraries. E-H. Fraction of each ENSEMBL transcript covered by transcripts assembled using indicated assembler:library pair (from A-D) was calculated. Image published in: Blower MD et al. (2013) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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