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Fig. 4. Rbpj mediates the repression exerted by FGF signalling on the RA pathway.We injected nLacZ mRNA and control (left panel) or the TP (middle panel) morpholinos into one blastomere of two-cell embryos and allowed them to develop to the tailbud stage. We treated embryos for 2 hours with SU5402. We detected β-galactosidase activity and mespa (A), cyp26a (B) or dlc (C) mRNAs by Red-gal staining and ISH. We also treated control morpholino-injected embryos with DMSO, stained them with the same probes, and we sorted DMSO and SU5402-treated embryos into 5 classes depending on their staining intensities (dlc, cyp26a) or the distance between the posterior tip of the embryo and the posterior limit of expression (mespa), and the right panels show the percentages of embryos within each of these classes. For injected embryos treated with SU5402, the right panels also shows the percentage of embryos with a staining intensity or a distance to posterior tip in the injected side above, equal to, and below (respectively green, yellow and red) that in the control side. We compared the distributions, between the classes, of the control and the other conditions by a chi-square test and we show the p-values. (A,B) dorsal views, anterior left, injected-side up, (C) lateral views of the injected side, anterior left. Image published in: Cibois M et al. (2013) © 2013. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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