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Figure 4. Pin1 stabilizes GFP-H1.1 and GFP-H1.5 dynamics. GFP H1.1 (A) or GFP-H1.5 (B) was expressed either in Pin1−/− cells or Pin1wt cells. FRAP experiments were performed to measure the dynamics of H1; each curve represents an average of ∼20 cells (total) in three independent experiments. The inset represents the same FRAP curve with the x-axis in log (time) to highlight changes in the earlier phases of the FRAP curve. Both H1.1 and H1.5 recover much faster in Pin1−/− cells as compared with Pin1wt cells. This trend was affirmed with a statistically significant increase in both t50 (C) and t90 (D) values in the presence of Pin1. Mathematical modeling of FRAP curves show that Pin1 causes a decrease in effective diffusion coefficient (E), a measure of the freely diffusing and low-affinity population, while at the same time causes increases residence time (F) of the high-affinity H1 population. Significance between Pin1−/− vs. Pin1wt was analyzed using unpaired t test (95% confidence interval). Notation for significance: *** if P value is < 0.001; ** if P is between 0.001 and 0.01; * if P is between 0.01 and 0.05. Image published in: Raghuram N et al. (2013) © 2013 Raghuram et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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