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Figure 2. An inducible expression system for Xenopus rods.(A) Schematic diagram of a Xenopus rod. In Xenopus, there is a daily synthesis of approximately 80 discs, and the previous disks are displaced apically. Thus, the distance of disks from the base of the OS is linearly related to the time after induction. (B) Schematic diagram of a XOP:G3U-Rho-mCherry system (left) and a XOP:Rho-mCherry constitutive expression system (right). Rod-specific expression is accomplished using the Xenopus rhodopsin promoter (XOP) driving transcription of G3. Dex treatment of animals transgenic for both XOP:G3 and pUAS:Rho-mCherry induces synthesis of Rho-mCherry that is transported and integrated into the rod outer segment (OS) disk membranes. Rods with XOP:Rho-mCherry express the Rho-mCherry constitutively. (C) Constitutive expression of XOP:Rho-mCherry transgene (top). There are two kinds axial variation of Rho-mCherrry expression in the OS: diurnal variation (middle) and long-term variation (lower). (D) Dex induction treatment paradigm 1. Tadpoles (St. 54) were treated with 10 µM Dex for 7 days and then sacrificed immediately before imaging. (E) Dex induction treatment paradigm II. Tadpoles (St. 54) received repetitive 3-day 10 µM Dex inductions (black boxes), each followed by a 5 day interval without Dex. Seven days after the last induction, retinas were explanted immediately before imaging. Image published in: Zhuo X et al. (2013) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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