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Figure 3. TGFB3C409Y does not activate TGF-β signaling. Constructs containing FLAG-tagged TGFB3WT and TGFB3C409Y were expressed in HEK293T cells and secreted FLAG-tagged proteins were immunoprecipitated under native conditions and normalized to anti-FLAG reactivity. Serial dilutions of these proteins were applied to HEK293T cells transfected with p3TP-Lux (TGF-β reporter) and pRL-CMV (normalizer). Error bars are standard error of the mean (N = 3). Image published in: Rienhoff HY et al. (2013) Copyright © 2013 Wiley Periodicals, Inc. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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