Xenbase Image ID: 129518

Figure 5. TGFB3C409Y does not disrupt BMP signaling during Xenopus gastrulation. Indicated amounts of synthetic mRNAs for human TGFB3, TGFB3C409Y and Xenopus Noggin were injected into Xenopus laevis embryos to assay perturbations of BMP signaling during gastrulation. The levels of C-terminal phosphorylated SMAD1 (pSMAD1) were examined by immunoblotting. SMAD1 and actin were used as loading controls. Pictured in the lower panel are intact embryos; the BMPR antagonist Noggin disrupts gastrulation while TGFB3C409Y does not. Image published in: Rienhoff HY et al. (2013) Copyright © 2013 Wiley Periodicals, Inc. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Experiment + Assay Source Phenotypes and Disease Xla Wt + nog + NF28 (whole-mount microscopy) Fig 5. row 2 column 3 Anatomical Phenotype abnormal gastrulation Larger Image Printer Friendly View

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