Xenbase Image ID: 129551

Figure 4. Model fitting reveals the stoichiometry of ADP activation of the KATP channel. (A) Assuming random assembly of WT and mutant subunits, the probability of occurrence of channels having exactly n WT subunits (Pn) follows a binomial distribution (equation shown at top). That probability Pn is shown as a function of the fraction of wild-type subunits (p) for each possible value of n as indicated above the curves. The label 2* corresponds to the probability of a channel having 2 adjacent (or 2 opposite) WT subunits. (B) Probability of a channel having n or more WT subunits calculated using the distributions of (A) (n indicated above the curves). The straight dotted line represents the probability of a WT channel if WT and mutant could not co-assemble. The symbols represent normalized ADP-activated current, calculated by normalization of the experimental data of Figure 3I. The rmsd of the experimental data from each model is: 0.327 (n = 4), 0.124 (n = 3), 0.119 (n = 2), 0.352 (n = 1), 0.031 (n = 2*), and 0.071 (no WT/mutant mixing; dotted straight line). Image published in: Hosy E and Vivaudou M (2014) Copyright © 2014 Hosy and Vivaudou. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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