Xenbase Image ID: 129556

Figure 6. Specificity of phenotype observed upon knock down of Mef2d.A. mef2d isexpressed on RNA level in heart tissue enriched explants at stages 24, 28, and 32 as revealed by qPCR. Expression is shown relative to gapdh. B. hMEF2D is expressed on protein level upon RNA injection into Xenopus embryo. β-tubulin served as loading control. Note the Mef2d antibody used does not recognize endogenous Xenopus Mef2d protein. C. Mef2d MO was unilaterally injected together with GFP or hMEF2D as indicated. Expression of cardiac marker genes myh6 and tnni3 was monitored at 28. Black arrowheads indicate reduced marker gene expression, white arrowheads highlight the rescued situation. D. A quantitative presentation of results is given. N: number of examined embryos; n: number of independent experiments; **, p≤0.01. Image published in: Guo Y et al. (2014) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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