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Figure 2. Binding affinities of anti-α1 and β2 scFvs measured using fluorescence polarization.Purified scFv proteins were incubated with 6 or 4 µM (α1 or β2, respectively), of FITC-conjugated target peptide. Changes in polarization were measured in millipolarization (mP) units and plotted against concentration of the scFv. The Kd value of one representative trial is shown. Error bars correspond to standard deviations for duplicate measurements. Image published in: Koduvayur SP et al. (2014) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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