XB-IMG-129849
Xenbase Image ID: 129849
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Distribution of anterior ventral (AV)-derived cells and dorsal marginal zone (DMZ)-derived cells in the embryos at the tailbud stage. (A) Beta-galactosidase (beta-gal) RNA (300 pg/embryo) was injected into the DMZ at the 4-cell stage. AV region at stage 13–14 or DMZ at stage 10+ was excised from the beta-gal-injected embryos and transplanted into stage 13–14 embryos. Host embryos were fixed at the tailbud stage (stage 32), stained with red-gal and subjected to whole-mount in situ hybridization analysis. A tailbud embryo (B, C) grafted with AV region (asterisk in B) and a tailbud embryo (D, E) grafted with DMZ region (asterisk in D), showing red-gal-positive cells (red) and peroxidase 2 (POX2)-positive cells (blue). Existence of double- positive cells (purple cells in C and E) in the lateral trunk region of the embryo revealed that a group of cells derived from the transplanted area migrated in the posterior direction and differentiated into POX2-positive leukocytes. Image published in: Tashiro S et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View |