XB-IMG-129850
Xenbase Image ID: 129850
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Fig. 3. Distribution of anterior ventral (AV)- and ventral blood island (VBI)-derived cells in the mesenchyme and peripheral blood at the early tadpole stage. The AV regions of Xenopus borealis embryos at stage 13–14 were excised and orthotopically transplanted into Xenopus laevis embryos. As a control experiment, the VBI regions of borealis embryos at stage 20–21 were transplanted. The host embryos were allowed to develop until appropriate stages and were fixed for histological examination to detect borealis (donor) cells. (A–F) Quinacrine-stained sections of AV-grafted animals (A–C) and VBI-grafted animals (D–F) at stage 46. A’–F’ are high magnification views of A–F. Borealis nuclei have brightly stained spots after staining with quinacrine (arrowheads in A’–F’). Significant numbers of borealis cells were observed in the mesenchyme near the epidermis (A, A’), the spinal cord (C, C’) and in the somite (B, B’) of AV-grafted animals. In contrast, many borealis cells were observed in the heart (D, D’), blood vessels (E, E’) and mesenchyme (F, F’) of VBI-grafted animals. Image published in: Tashiro S et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View |