Xenbase Image ID: 129851

Fig. 4. Expression of peroxidase 2 (POX2) in dorsal marginal zone (DMZ) and ventral marginal zone (VMZ) explants. (A–I) Whole-mount in situ hybridization analysis showing the expression of POX2 (B, C, H, I) and α-globin (E, F) in DMZ (B, E, H) and VMZ (C, F, I) explants. Explants were harvested at stage 30 (B, C, E, F) or at stage 37/38 (H, I). Control whole embryos (w. e) are also shown (A, D, G). (J) Reverse transcription-polymerase chain reaction (RT-PCR) analysis. Explants and the control whole embryos (w. e) were harvested at stage 30 or stage 37/38 and processed for the RT-PCR analysis to show the expression of POX2 and EF1α. The results demonstrated that both the DMZ and VMZ explants contain leukocyte precursor cells, but only the cells in DMZ explants differentiate at the early stage (stage 30). Image published in: Tashiro S et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

Return to previous page