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Induction of peroxidase 2 (POX2) in animal cap explants. The animal cap was excised from a mid-blastula embryo, and cells were dissociated in a Ca2+-free medium for 20 min and then reaggregated in the medium containing activin, as described previously (Ariizumi et al. 2003). Aggregates were further cultured until stage 33 and fixed to process for reverse transcription-polymerase chain reaction (RT-PCR) and whole- mount in situ hybridization analyses. (A) Expression of POX2, tnIc (a heart marker), transferrin (a liver marker) and EF1α (loading control) was examined by RT-PCR analysis. POX2 expression was strongly induced in the explants treated with 100 ng/mL activin. The explants also expressed tnIc but did not express transferrin. (B–E) Expression of POX2 and tnIc was examined by whole-mount in situ hybridization analysis. Image published in: Tashiro S et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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