XB-IMG-129869
Xenbase Image ID: 129869
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Fig. 1. Generation of transgenic animals expressing Venus. A: Structure of the constructed transgene. The transgene CAG- Venus is composed of the fluorescence reporter gene Venus and the rabbit -globin polyA signal sequence under the control of the CAG promoter. Another transgene, XCar-GFP3, is composed of the fluorescence gene GFP3, the SV40 polyA signal sequence, and the Xenopus cardiac actin promoter (Mohun et al., 1986). B: Fluorescent analyses of the transgenic animals. Images of transgenic tadpoles carrying the CAG-Venus (upper animal in a) or the Xcar-GFP3 transgene (lower animal in a, and b) at stage 50 were captured as both brightfield (left panels) and fluorescence (right panels) under the microscope. Image published in: Sakamaki K et al. (2005) Copyright © 2005. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View |