XB-IMG-129873
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Fig. 4. (Part 2) Morphological and histological analyses of Venus fluorescence in the tissues of CAG- Venus transgenic frogs.
K-L: Fluorescent analysis of Venus pro- teins in a variety of tissues dissected from transgenic animals; images of the .... ovaries (K), and testis (L) were captured in brightfield (left panels) and fluorescence (right panels) under the microscope. M,N: Histological analysis of the reproductive organs; sections prepared from specimens of the transgenic ovary (M) and testis (N) were taken as fluorescence images and then stained with hematoxylin and eosin and photographed under the microscope. O, immature oocyte; Sc, spermatocytes; Sg, sper- matogonia; Sp, sperm. O–T: Immunohisto- chemical analysis of the brain; as indicated in O, transverse sections derived from the olfac- tory bulb (P), cerebrum (Q), diencephalons (R), mesencephalon (S) and hypophysis (T) of the brain were immunostained with anti–green flu- orescent protein antibody (red) and counter- stained with Hoechst (blue). cp, choroid plexus; e, ependymal cell; gl, glomerulus; gr, granule cell; h, hypophysis; lv, lateral ventricle; m, mitral cell; n, nerve cell; tev, tectal ventricle; thv, third ventricle. Scale bars 25 m in M,N, 50 m in O–T.
Image published in: Sakamaki K et al. (2005) Copyright © 2005. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View |