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Fig. 5. Transplant engraftment with the brain from transgenic animals. A: Schematic diagram displaying the protocol for brain transplanta- tion. Wild-type tadpoles (n  15) at stages 55–58 were ectomized by removing the anterior one third of the forebrain, and a fragment of the whole brain from CAG-Venus transgenic tad- poles (stages 56–58) was implanted. After the transplantation, the recipient larvae were kept in water for 1, 3, and 4 weeks. B–E: Morpho- logical analysis of the implanted animals; heads of the implanted tadpoles (B,C) and frogs (D,E) were photographed in the light (left), fluorescent (middle), and magnified (right) fields under the microscope at 1 week (B,C), 3 weeks (D), and 4 weeks (E) after surgery. Arrows indicate an en- grafted area. animals (data not shown). Thus, we embryo to adult stage. Consequently, are currently attempting to visualize we believe CAG-Venus transgenic

Image published in: Sakamaki K et al. (2005)

Copyright © 2005. Image reproduced with permission of the Publisher, John Wiley & Sons.

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