Xenbase Image ID: 129905

Fig. 3. Whole-mount in situ hybridization of Xotx2 during Xenopus gastrulation. (A) Vegetal view of a stage 10 embryo. (B) Dorsovegetal view of a stage 12 embryo. (C-E) Dorsal portion of midsagittal sections of whole-mount in situ hybridized stage 10+, 10.25, and 12 embryos, respectively. Anterior is to the right in all panels except F. Xotx2 staining in C is confined to deep mesoderm, while in D and E the expression in ectoderm is apparent (arrowheads in D indicate a region of nuclear ectodermal Xotx2 staining). In E Xotx2 expression in both ectoderm and mesoderm are approximately in register. Note the ectodermal thickening marking the presumptive anterior neurectoderm (arrow). The anterior border of ectodermal staining extends further anteriorly into presumptive cement gland (see also Jamrich and Sato, 1989). (F) Xotx2 is induced in ventrolateral mesoderm expressing goosecoid. Single C-tier blastomeres were coinjected with goosecoid RNA and the lineage tracer LRD at the 32-cell stage. At the early gastrula stage, embryos containing LRD at a position greater than 120° away from the dorsal midline were subjected to whole-mount in situ hybridization for Xotx2. A vegetal pole view is shown and the primary and secondary sites of Xotx2 expression are indicated. Image published in: Blitz IL and Cho KW (1995) Copyright © 1995. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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