Xenbase Image ID: 129908

Fig. 6. Xotx2 induces cement gland marker expression in isolated animal caps. (A) RT-PCR analysis of RNA derived from stage 21 embryos (lane 1) and animal caps isolated from uninjected (lane 2) and Xotx2-injected embryos (lane 3) at stage 8 blastula, and developed until sibling embryos reached stage 21. RNA was subjected to RT-PCR analysis using oligonucleotides specific for the cement gland marker XAG1 and histone H4. (B) RT-PCR analysis of RNA derived from stage 21 embryos (lane 1) and animal caps isolated from uninjected (lane 2), noggin-injected (lane 3) and Xotx2- injected embryos (lane 4) at stage 8 blastula, and developed until sibling embryos reached stage 21. RNA was subjected to RT-PCR analysis using oligonucleotides specific for Xotx2, N-CAM, and histone H4. Lanes marked E, A, N, and X contain PCR products from normal embryos, animal (uninjected control) caps, noggin- injected caps, and Xotx2-injected caps, respectively. Image published in: Blitz IL and Cho KW (1995) Copyright © 1995. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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