Xenbase Image ID: 129953

Figure 3. Deep Cells Display Elevated Levels of Lrp6 and Wnt/b-Catenin Signaling (A) Scheme and immunofluorescence confocal microscopy of isolated single outer blastomere from the animal region of a 64-cell embryo. Endogenous Lrp6 and b1-integrin were stained as indicated. Scale bar represents 50 mm; pig, pigment. See also Figure S3. (B) Scheme and western blot analysis of dissected ectodermal cell layers (stage 10+). Embryos were injected with the indicated MOs or mRNAs. (C–F) Luciferase reporter assays for the indicated signaling pathways and qRT-PCR analysis of the indicated genes from dissociated blastomeres (C and D) or dissected ectodermal cell layers (E and F). rlu, relative light units. Error bars, SD. Experiments were typically carried out at least three times with similar results. In (A), more than 50 blastomeres were analyzed. See also Figure S3. Image published in: Huang YL and Niehrs C (2014) Copyright © 2014. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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