Xenbase Image ID: 129954

Figure 4. Lrp6 Asymmetry and Wnt/b-Catenin Signaling Regulate Ciliated Cell Specification (A–F) Immunofluorescence microscopy of ciliated cells stained for acetylated-tubulin in neurulae. Dorsal view of whole embryos (A–F) or confocal images of cryosections from the flank of the embryo (A0–F0) are shown. Embryos were unilaterally injected with the indicated mRNAs or MOs (left side, control; right side, injected). Upper panels: magnifications of the boxed areas; arrowheads, ciliated cells. Experiments were typically carried out three times with similar results. (G) Quantification of ciliated cells in (A)–(F) showing relative Ac-tubulin positive cell number normalized to the control side; n > 10 embryos per condition and >500 Ac-tubulin positive cells for each uninjected embryo side. Error bars, SD. (H–O) qRT-PCR analysis of XK70b, inca B, E-keratin, and a-tubulin of dissected ectodermal cell layers injected with the indicated mRNAs or MOs. Experiments were typically carried out three times with similar results. See also Figure S4. Image published in: Huang YL and Niehrs C (2014) Copyright © 2014. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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