Xenbase Image ID: 129955

Figure S1,A & B related to Figure 1. Confirmation of speci- ficity of Lrp6 and phospho-Lrp6 antibody staining (A) Scheme showing cell fates of Xenopus blastula/gastrula ectodermal cells. The outer ectodermal layer consists of epithelial cells (yellow), which are apico- basally polarized. The inner layer consists of deep cells which are unpolarized. On the dorsal side the epithelial cells give rise to secondary neuronal precursors and the deep cells to primary neurons. On the ventral side the epithelial cells give rise to goblet cells and the deep cells to multiciliated cells. The midblastula stage is shown in section to reveal the blastocoel. (Modified from K. Kalthoff, Analysis of Biological Development, 2d ed., McGraw-Hill, 2001) (B,C) Confocal immunofluorescence microscopy of moru- lae and blastulae stained for endogenous Lrp6 or phospho-Lrp6 (pLrp6). (C) Embryos were maternally depleted for Lrp6. Embryos were cryo-sectioned and stained for Lrp6 or pLrp6 (green) together with β1-integrin (red). Nuclei are marked in blue (Hoechst). Scale bar, 30μm. Image published in: Huang YL and Niehrs C (2014) Copyright © 2014. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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