Xenbase Image ID: 129963

Fig. 4. miRNAs regulate mTORC1 pathway activity. (A–D′) pAkt and pS6 immunofluorescence analysis of uninjected controls and xDicer-MO–injected embryos at stage 42. A, B, C, and D show pAkt or pS6 staining only; A′, B′, C′, and D′ show merged images with DAPI (blue) and ECL (green) to visualize nuclei and proximal tubules, respectively. (E–G′) pS6 immunofluorescence comparing LLC-PK1 proximal tubular cells lipofected with scr-MO or sDicer-MO in the presence or absence of 0.1 μM Insulin or 0.01 nM Igf2. (H and I) Western blot analysis of LLC-PK1 cells lipofected with decreasing amounts of scr-MO or sDicer-MO in the presence of 0.01 nM or 0.1 nM recombinant Igf2 protein. α-Actin serves as a loading control. Image published in: Romaker D et al. (2014) Copyright © 2014. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Experiment + Assay Source Phenotypes and Disease Xla Wt + dicer1 MO + NF42 (immunohistochemistry) fig.4.b Xla Wt + dicer1 MO + NF42 (immunohistochemistry) fig.4.d Anatomical Phenotype abnormal protein phosphorylation Larger Image Printer Friendly View

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