Xenbase Image ID: 129976

Figure 1. Cnn2 Is Expressed in Migratory NCCs in Frogs and Chicks (A–F) Frog. Cnn2 mRNA localization is shown in delaminating (A) and early migrating NCCs (B and C). Comparison with Xsnail (D), Xslug (E), and Xtwist (F) demonstrates that Cnn2 mRNA was found during early stages of emigration. Transcription was attenuated as cells progressed ventrally (cf. the strength of signals in proximal and distal NCCs in C and F). Note that Cnn2 remained to be expressed in a subpopulation of mandibular, hyoid, and branchial NCCs (open arrowheads). Additional sites of Cnn2 mRNA localization were found in the notochord (A0 –C0 ), heart (asterisk in C), somites, pronephros, and pronephric duct (arrowheads in C). (A0 –C0 ) Histological transverse vibratome sections (levels indicated in A–C). Frontal views in (A), (B), (D), and (E); lateral views (anterior to the left) in (C) and (F). ba, branchial; hy, hyoid; ma, mandibular; no, notochord. (G–I) Chick. Ccnn2 expression in migratory cranial and cervical NCCs (arrowheads) at HH10 (G), HH11 (H), and HH12 (I). Embryos are shown in dorsal view. Costaining with HNK1 antibody (H, red) reveals a partial overlap, demonstrating expression during early stages of emigration, as the HNK1 epitope only arises at a distance from the neural tube. (G0 and H0) Cranial and cervical transverse vibratome sections at levels indicated in (G) and (H). Krox20 (I, blue) costaining, which marks r3 and r5, demonstrates expression in migratory streams at r2, r4, and r6, with continued and strong expression in the hyoid stream (r4). hb, hindbrain; mb, midbrain; ov, optic vesicle; pm, paraxial mesoderm; r, rhombomere. See also Figure S1. Image published in: Ulmer B et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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