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Figure S3. Xcnn2 Knockdown Rescues dnWnt11, Related to Figure 3 (A–D) Embryos were injected unilaterally into animal blastomeres at the 4-8 cell stage and analyzed for NCC migration by Xtwist WMISH at stage 26. NCC migration (A) was compromised upon Xcnn2 knockdown (B) and dnWnt11 injection (C), and rescued by co-injection of Xcnn2MO and dnWnt11 mRNA (D). Histological sections (A′ –D′) at the levels indicated (dashed lines) demonstrated equal (A′, D′) and unequal (B′, C′) dorso-ventral extension of Xtwist-positive NCC. (A″ –D″) uninjected control side. (E) Xcnn2MO suppressed Y27632-enhanced NCC migration in explants. Xcnn2 knockdown did not significantly alter NCC migration compared to wild-type explants (top). Rock inhibitor Y27632 induced excess migration, which was suppressed by parallel knockdown of Xcnn2 (bottom). (F–H) Xcnn2 polarization (F) is lost upon inhibition of ROCK (G) is mimicked by a deletion of potential ROCK- target sites in a Xcnn2 deletion construct which lacks clik repeats ((Xcnn2Δclik; H; cf Figure S1). Image published in: Ulmer B et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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