Xenbase Image ID: 130022

Fig. 4. mRNA injection of Smed-βcatenin1 but not Smed-βcatenin2 induces a secondary axis in Xenopus embryos. (A,B) Xenopus embryos injected with Smed-βcatenin1 (A) or Smed-βcat2 RNA (B), showing Otx2 (forebrain, red arrow), Krox20 (rhombomeres 3 and 5, green arrows) and Cad3 (spinal cord, white arrows) expression, along with muscle staining with the antibody 12/101 (brown signal). A schematic representation of the structure of each β-catenin is shown under the corresponding image. GSK3-binding domain and adhesion domains are shown with red and blue boxes, respectively. (C-H) Neurula (C-F) and tailbud (G,H) stage embryos injected with GFP-tagged constructs for both Schmidtea mediterranea β-catenins. (I,J) Smed-βcatenin1 but not Smed-βcatenin2 is found preferentially in the cell nucleus. (K) Quantification of the secondary axis obtained after each Smed-βcatenin injection (Xenopus β-catenin was used as a control). Image published in: Iglesias M et al. (2008) Copyright © 2008. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue cdh3.S b-cadherin, c-cadherin, cdh3-a, cdh3-b, cdhc-A, cdhp, EP-cadherin, hjmd, P-cadherin, pcad, U-cadherin, xb-cad, XB-cadherin, XBcad X. laevis Throughout NF stage 26 spinal cord egr2.L EGR-2, Krox-20, krox20, XKr20, XKrox-20 X. laevis Throughout NF stage 26 rhombomere R3 rhombomere R5 otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 26 forebrain Image source: Published Larger Image Printer Friendly View

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