Xenbase Image ID: 130150

Fig. 2 Expression and loss of function reveal a role for Rai1 in craniofacial and brain development. (A) In situ hybridization of rai1 mRNA in Xenopus tropicalis. (i) Dorsolateral view at stage 18–19 revealing labeling throughout the developing nervous system. (ii) Lateral view of stage 23, revealing rai1 in the presumptive migrating neural crest (arrows) and eye. (iii) Lateral view of stage 26 revealing rai1 in the presumptive migrating neural crest (arrows). (iv) Lateral view of stage 33 revealing rai1 in the branchial arches (arrows) eye, optic vesicle (ov) and muscle segments (ms). (v) Dorsal view at stage 33 revealing rai1 in the central nervous system (CNS). (vi) Lateral view at stage 41 revealing rai1 throughout the head. (vii) Transverse sections through the head at stage 41 revealing that rai1 is primarily located outside the CNS in the tissues of the face at this stage. Abbreviations, np; neural plate, ms; muscle segments, ov; otic vesicle, cg; cement gland, CNS; central nervous system. (B) Decreased function of Rai1 results in dramatic effects on Xenopus laevis development including craniofacial defects. (i) Lateral view of representative embryo at stage 42 injected with control morpholino (MO). (ii) Lateral view of representative embryo at stage 42 injected with 5 ng of MO. Defects include digestive tract, shortened tail and smaller head. (iii) Lateral view of representative embryo at stage 42 injected with 10 ng of Rai1 MO. There are severe defects in digestive tract, tail and head development. Image published in: Tahir R et al. (2014) Copyright © 2014. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue rai1.S smcr, sms X. laevis Throughout NF stage 18 neural plate chordal neural plate pre-chordal neural plate rai1.S smcr, sms X. laevis Throughout NF stage 23 brain neural crest central nervous system head optic vesicle presomitic mesoderm neural tube ventral blood island otic placode rai1.S smcr, sms X. laevis Throughout NF stage 33 and 34 brain forebrain otic vesicle eye central nervous system head ventral blood island pharyngeal arch mandibular arch hyoid arch branchial arch pronephric kidney somite trunk somite tail somite cranial neural crest rai1.S smcr, sms X. laevis Throughout NF stage 41 brain eye head pharyngeal region pharyngeal mesenchyme head mesenchyme spinal cord retina Image source: Published Larger Image Printer Friendly View

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