Xenbase Image ID: 130154

Supplementary Figure 2. A,B) Xenopus laevis in situ hybridization using a probe generated from the 3’UTR of the Xenopus tropicalis rai1 sequence. The region shares a 66% nucleotide similarity with the same region of the X. laevis rai1 gene and showed a highly similar staining pattern in X. laevis embryos. The probe sequence did not match any other sequence in either Xenopus species with identity of greater than 20% or with a stretch of nucleotides longer than 50. C,D) The sense or negative control probe show no significant staining pattern. A blue background was necessary to show contrast with the starkly white embryos. Image published in: Tahir R et al. (2014) Copyright © 2014. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue rai1.S smcr, sms X. laevis Throughout NF stage 28 brain otic vesicle migratory neural crest cell anterior branchial crest posterior branchial crest cranial neural crest mandibular crest hyoid crest midbrain-hindbrain boundary olfactory placode pronephric mesenchyme intersomitic region neural tube forebrain midbrain hindbrain Image source: Published Larger Image Printer Friendly View

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