Xenbase Image ID: 130155

Supplemental Figure S3) Establishing Rai1 morpholino specificity. A) A second translation blocking morpholino (Rai1 MO2) results in very similar phenotypes as the first morpholino used in this study. (i) Lateral view of representative embryo at stage 42 injected with control morpholino (Cont MO). (ii) Lateral view of three representative embryos at stage 42 injected with 5ng of Rai1 MO2. Defects include malformed digestive tract, shortened tail and smaller head. (iii) Lateral view of representative embryo at stage 42 injected with 10ng of Rai1 MO. There are more severe defects in the digestive tract, tail and head. (iv) Frontal view of the face of a control embryo at stage 42. (v) Frontal view of the face of an embryo injected with 5ng of Rai1 MO2. The face is narrower and the mouth rounder as in embryos injected with the same amount of original Rai1 morpholino. B) (iv) Transverse section of stage 20 embryo injected with control MO and labeled with antibodies to human RAI1. (v) Transverse section of stage 20 embryo injected with Rai1 MO and labeled with human Rai1 antibodies revealing a noticeable decrease in immunofluorescence. (vi) Quantification of Rai1 immunofluorescence by pixel intensity using photoshop software. Results show a 2.16 fold decrease that is significant (t-test, p=0.000116, n= 10). Abbreviations, np; neural plate, nc; notochord, cg; cement gland. Note in this and supplemental methods- The pixel intensity from 10 embryos from the two experiments was pooled and averaged. Image published in: Tahir R et al. (2014) Copyright © 2014. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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