XB-IMG-130209
Xenbase Image ID: 130209
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Fig. 6. Comparison of the expression of Zic3 with the accumulated location of Ca2 transients. Representative aequorin-loaded (n 5) (A) untreated control
and (B) BAPTA-AM treated explants to show (ii) pseudo-3D profile of accumulated increase in [Ca2 ]i during the 8 h of data acquisition that corresponds
to gastrulation and (i) the subsequent expression pattern of Zic3, determined by in situ hybridization in the same explant. The Ca2 increase above the
background (5 photons) is outlined in black in (Ai) to show that this region correlates with the pattern of Zic3 expression. (C) Representative examples of
untreated control and BAPTA-AM-treated explants to show Zic3 expression (n 38, 4 independent experiments). E and M are ectoderm and mesoderm,
respectively. Image published in: Leclerc C et al. (2003) Copyright © 2003. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |