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Fig. 3. MAK (hunk) is an activator of JNK. JNK activity was analyzed in embryonic lysates by in vitro phosphorylation of GST-Jun(1-135). Four-cell embryos were injected marginally four times with GFP, MAK or MAK-KD RNA (1.5 ng per injection), or δN-Fz8 RNA (0.5 ng per injection) as a positive control. Lysates were collected at stage 14 for JNK activity determination by western analysis with anti-phospho-Jun antibodies. Assays were carried out in duplicates, with 10 embryos in each experimental group. Anti-GST and anti-β-tubulin antibodies indicate loading. Image published in: Kibardin A et al. (2006) Copyright © 2006. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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