Xenbase Image ID: 130269

Fig. 4. MAK (hunk) interacts with Frizzled and Dishevelled. (A) Fz3 RNA recruits MAK to the cell membrane in animal pole cells. xMAK-GFP RNA was injected either with membrane-targeted dsRed RNA or with dsRed and Fz3 or Fz2 RNAs and GFP fluorescence was visualized in animal pole cells isolated from injected embryos at stage 10. xMAK-GFP is distributed in a punctate cytoplasmic pattern. Membrane-targeted dsRed RNA allows visualization of the cell membrane. Upon Fz3 RNA coexpression, xMAK-GFP is recruited to the cell membrane. The bottom right panel shows lack of effect of Fz2 RNA on xMAK-GFP localization (compare with top right panel). Bars on top left and top right panels are 5 μm. (B) MAK co-immunoprecipitates with Dsh. Lysates of embryos injected with Myc-MAK RNA (2 ng) and/or with HA-Dsh RNA (4 ng) into each cell of four-cell embryos were collected at stage 10 and immunoprecipitated with anti-HA antibodies. Western analysis with anti-Myc antibodies reveals a complex of Dsh and MAK. (C) In vitro phosphorylation of Dsh by MAK. Autoradiography (upper panel) reveals phosphorylation of in vitro translated Myc-Dsh that was co-immunoprecipitated with Myc-MAK or Myc-MAK-KD from in vitro translated lysates. Lower panel shows protein levels. Image published in: Kibardin A et al. (2006) Copyright © 2006. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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