Xenbase Image ID: 130273

Figure S1. Identification and Characterization of Xenopus CST (A) Predicted genomic locus structure of Xenopus Cstα and Cstβ (5' to 3', not to scale). Exons are shown in boxes with the corresponding size given in basepairs. Exons in gray depict those containing the zinc finger repeats, and the sizes of intervening introns are indicated beneath each intron. Alternative splicing of the 5' regions is also indicated. (B,C) Table showing the evolutionary conservation of the full length CST amino acid sequence (B) and the zinc finger repeats (repeats 1-4) (C). The percentage of identical amino acids (identity) and the percentage of conservative substitutions (similarity) are given for comparison between CST proteins of X. tropicalis, hCST (human), mCST (mouse), predicted gCST (chicken), and dCAS (Drosophila). (D) Syntenic relationship between vertebrate Cst genomic loci using Metazome blast analysis. (E) Western blot analysis of CSTα-V5 and CSTβ-V5 in vitro translation. Both CST proteins run at the predicted size of 148 kDa. (F,G) CSTα and CSTβ cellular localization. Transverse confocal images of CSTα-V5 and CSTβ-V5 injected Xenopus embryos at Stage 32. Histological sections were stained with a V5 antibody (Red) to visualize the CST proteins and with DAPI (Blue) to identify the nucleus. Scale bar indicates 10μm. Image published in: Christine KS and Conlon FL (2008) Copyright © 2008. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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