XB-IMG-130277
Xenbase Image ID: 130277
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Figure S5. Morpholino Design and Phenotype
(A) Position of the Cst splice junction morpholinos relative to the pre-mRNA transcripts
targeting the donor the exon 8 (ex8D MO) and the acceptor of exon 9 (ex9A MO),
referred collectively as CstMO. (B) Position of the Cst-5′ UTR morpholinos (red) relative
to the Cstα and Cstβ cDNA transcripts. (C-D) Whole mount antibody staining with anti-
MHC of Stage 32 control MO (C) and Cstα/β MO (D) embryos (ventral view) indicating
an identical cardia bifida phenotype is obtained with both the CstMO (splice MO) as the
Cstα/β MO (5′ UTR MO). Scale bar: C = 100 μm. Image published in: Christine KS and Conlon FL (2008) Copyright © 2008. Image reproduced with permission of the Publisher, Elsevier B. V.
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