Xenbase Image ID: 130282

Figure 2 Co-expression of GluK2-mEGFP and GluK5-mCherry (A) Images from a representative movie. The circles indicate stationary spots that were observed in both the green mEGFP and the red mCherry channel. The bar graph shows the fractions of green-only, colocalizing and red-only spots for a total of 599 spots. (B) The co-expression experiment with GluK2-mEGFP and GluK5-mCherry allows to count the bleaching step distribution of GluK2 subunits colocalizing with GluK5. Left: One example trace with two mEGFP bleaching steps is shown. Right: Bleaching step analysis of 124 colocalizing spots. The red line gives the binominal distribution expected for two subunits with a probability of 0.80 for a single mEGFP to be fluorescent. The error bars represent the counting uncertainty. (C) Experiment with GluK2 and GluK5-mEGFP, which allows to count the number of GluK5 subunits per complex. Left: One example trace is shown. Right: The number of bleaching steps from 932 receptors agrees well with the binominal distribution expected for two subunits (red line, probability of 0.80 for a single mEGFP to be fluorescent). Image published in: Reiner A et al. (2012) Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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