XB-IMG-130372
Xenbase Image ID: 130372
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Figure 2. Identification of the photoreceptor-specific R9AP isoform in Xenopus. (A) Phylogeny tree of R9AP protein sequences built using the Neighbor-Joining method. Accession nos. are as follows: bovine, AAM67417; chicken, NP_989836; chimpanzee, XP_512566; dog, XP_855518; human, NP_997274; macaque, XP_001087093; mouse, NP_665839; pufferfish, CAF92756; rat, XP_001079782; zebrafish, XP_687644. Xenopus tropicalis R9AP1 is deduced from genome v4.1, scaffold 94 (920566:921276), and X. tropicalis R9AP2 is deduced from genome v4.1, scaffold 761 (402303:408685). (B) RT-PCR amplification of xR9AP1 and xR9AP2 transcripts from Xenopus retina; omission of reverse transcription (RT) in parallel samples served as a negative control for DNA contamination. (C) Western blot of Xenopus retina extract probed with the antibody specific to xR9AP2. Lane T, total extract; lane S; soluble protein fraction; lane M, membrane fraction. Note that the xR9AP2 band is a doublet, which reflects the previously documented phosphorylation of R9AP in photoreceptors (Martemyanov et al., 2003). (D) xR9AP2 coimmunoprecipitates with RGS9-1 from Xenopus retina. Beads coated with sheep IgG were used for the negative control. Image published in: Baker SA et al. (2008) © 2008 Baker et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View |