Xenbase Image ID: 130401

Figure 2. Mad GSK3 Mutants Mimic Wg Overexpression.(A–C) Additional bristles are induced in the wing margin by MGM driven by scalloped-Gal4. Arrows indicate chemosensory bristles and arrowheads stout mechanosensory bristles. Insets show that the number of Senseless-expressing bristle precursors in wing imaginal discs is increased by MGM, but not MWT.(D–G) Expression domains of sd-Gal4 driver, Wg protein, Armadillo stabilized by Wg (expression in the proveins is noted), and Senseless in wing discs. (H and I) Senseless protein expression was inhibited in Mad RNAi clones marked by GFP. (J–O) Quantitative RT-PCR of wing discs showing that MGM increased both a Wg target gene (senseless) and Dpp target genes (spalt and optomotor blind), while not affecting wg or hh levels. dpp was inhibited by MGM RNA. Samples were normalized for rp49, except for the inset in L in which Gal4 mRNA was used. (P and Q) Clonal overexpression of MGM does not change Wg levels. (R and S) MGM clone in the anterior margin causes ectopic expression of Senseless within the clone (inset). (T) Overexpression of MGM in clones marked by yellow induced duplications of the wing margin (n = 24). Yellow arrowheads indicate ectopic margins and a black arrowhead the wild type one. (U) Knockdown of Mad in clones partially eliminates the wing margin In this large clone the remaining margin bristles are yellow (y). Image published in: Eivers E et al. (2009) Eivers et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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