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Figure 1. Co(III)-Ebox alleviates murine Snai1-mediated E-cadherin promoter repression.(A) Chemical structures; Co(III) Schiff base complex, Co(III)-sb; Ebox duplex oligonucleotide, Ebox; Co(III)-DNA conjugate with 2-base pair substitution in the Ebox region; Co(III)-EboxMut; Co(III)-DNA conjugate targeted to Snail factors, Co(III)-Ebox. (B) E-cadherin reporter gene activity in MCF7 cells expressing MSnail treated with Co(III)-Ebox from 0 to 15 nM. Data are represented as means ± s.e.m., n = 4. T-tests determined statistical significance from controls of 10 nM (P = 0.0034), 12.5 nM (P = 0.0060) and 15 nM (P = 0.0109) (*). (C) E-cadherin reporter gene activity in MCF7 cells expressing MSnail treated with 15 nM Co(III)-sb, Ebox, Co(III)-EboxMut or Co(III)-Ebox. Data are represented as means ± s.e.m., n = 4. T-tests determined statistical significance from controls of Co(III)-EboxMut (P = 0.0214) and Co(III)-Ebox (P = 0.0035) (*). Co(III)-Ebox to be significantly different from Co(III)-EboxMut (P = 0.0008) (**). (D) E-cadherin reporter gene activity in MCF7 cells transfected with pCS2+ (vector) treated with 15 nM Co(III)-sb, Ebox, Co(III)-EboxMut or Co(III)-Ebox. Data are represented as means ± s.e.m., n = 4. T-tests determined no statistical significance between the means. (E) MCF7 cell viability after treatment with Co(III)-Ebox at indicated concentrations after 24 h. Data are represented as means ± s.e.m., n = 3. T-tests determined no statistical significance between the means. Image published in: Harney AS et al. (2012) Harney et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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